Webrecommend 1x siRNA buffer (diluted from 5x siRNA buffer – Dharmacon Products Cat. #B-002000-UB-100). RNase-free water (for short-term storage) is also appropriate for resuspension of concentrated stocks (such as 20-100 μM). Alternatively, an RNase-free buffer (pH 7.3-7.6) may be used such as PBS (Fisher Cat. #NC9826748) WebResuspend siRNA or Stealth RNAi duplexes in DEPC-treated water according to the chart in order to make a 20 µM solution. The RNA oligo was dried down from a buffered solution. Resuspension to 20 µM will reconstitute the buffer to 10 mM Tris-HCl, pH 8.0, 20 mM NaCl, 1 …

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WebThe stock concentration for the siRNA library plates designated for screening is 1 µM. All of the siRNAs are stored in 1x Dharmacon siRNA buffer (20 mM KCl, 6 mM HEPES-pH … WebJul 14, 2016 · The resuspension of siRNA pellets was done in 1X siRNA Buffer (Thermo Scientific, Dharmacon). The working aliquots (20 μM) were then stored at −20 °C. In this report, we used a final siRNA ... WebAug 16, 2016 · In the primary screen, Dharmacon siGENOME SMARTpool siRNA library (GE Dharmacon, Lafayette, CO) was used. To prepare screening plates, the siRNAs in each well were suspended in 1 × siRNA buffer (Thermo Fisher Scientific, Waltham, MA) and 2.5 pmol siRNA (2.5 μL/well) was dispensed into black, clear bottom, 384-well … porch revit family